Product Details
Catalog Number: MO15120
Applications: WB, IHC
Type: Mouse IgG
Immunogen: Hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, E. coli-derived recombinant human ERK3 (rhERK3; aa 344 - 721; Accession # NP_002739).
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human
Entrez: 5597
UniProt: Q16659
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$255.00Add to Cart

Extracellular signal-regulated kinase 3 (ERK3) is a serine/threonine protein kinase also known as mitogen-activated protein kinase 6 (MAPK6) and p97MAPK. Unlike ERK1 and ERK2, ERK3 lacks the phosphoacceptor sequence T-E-Y in its activation loop. This kinase also possesses a unique C-terminal extension that is missing in traditional MAPKs. Highly unstable, ERK3 is rapidly degraded following N-terminal ubiquitination.

ERK3 is involved in various cellular proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals making it useful for the study of processes like angiogenesis, neurogenesis and tumorigenesis. The activation of ERK by proinflammatory stimuli may play important roles in innate immune responses and autoimmunity.

Images

Extracellular signal-regulated kinase 3 (ERK3) staining of paraffin-embedded human breast cancer tissue sections using 8 µg/mL mouse anti-human ERK3 monoclonal antibody. Tissues were stained using anti-mouse HRP-DAB (brown) and counterstained with haematoxylin (blue).

Extracellular signal-regulated kinase 3 (ERK3) staining of paraffin-embedded human breast cancer tissue sections using 8 µg/mL mouse anti-human ERK3 monoclonal antibody. Tissues were stained using anti-mouse HRP-DAB (brown) and counterstained with haematoxylin (blue).

Detection of ERK3. Lysate from human U937 cells was resolved by SDS-PAGE, transferred to an Immobilon-P membrane, and immunoblotted with 1 μg/mL mouse anti-ERK3, as described in Protocol on Datasheet. A 15 second exposure to film is shown

Detection of ERK3. Lysate from human U937 cells was resolved by SDS-PAGE, transferred to an Immobilon-P membrane, and immunoblotted with 1 μg/mL mouse anti-ERK3, as described in Protocol on Datasheet. A 15 second exposure to film is shown