Product Details
Catalog Number: MO15132
Applications: WB
Type: Mouse IgG
Dilutions: Western Blot: 0.5 ug/ml
Immunogen: E. coli-derived recombinant human Caspase-7
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Protein A purified
Format B: liquid
Species Reactivity: Human, Mouse
Entrez: 840
UniProt: P55210
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$359.00Add to Cart

Caspase-7 (Cysteine-aspartic acid protease 7/Casp7; also CMH-1, ICE-LAP3 and Mch3) is a 32 kDa member of the peptidase C14A/IL-1 beta -converting family of enzymes. It is widely expressed, except in brain, and is best known as an integral component of the apoptotic cascade. Caspase-7 is considered to be an executioner caspase, as a downstream mediator of apoptotic-associated proteolysis. Upon activation, Caspase-7 is known to utilize a Cys residue to cleave multiple substrates, including PARP, procaspase 6, Gas2 and calpastatin.

Images
Western Blot

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line untreated (-) or treated (+) with 1 μM staurosporine (STS) for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse Caspase‑7 Monoclonal Antibody, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. Specific bands were detected for Caspase‑7 precursor and large subunit at approximately 38 and 20 kDa (as indicated).

Western Blot

Western blot shows lysates of Jurkat human acute T cell leukemia cell line and DA3 mouse myeloma cell line untreated (-) or treated (+) with 1 μM staurosporine (STS) for the indicated times. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse Caspase‑7 Monoclonal Antibody, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. Specific bands were detected for Caspase‑7 precursor and large subunit at approximately 38 and 20 kDa (as indicated).