Product Details
Catalog Number: MO15136
Applications: WB
Type: Mouse IgG
Dilutions: Western Blot: 1 ug/ml
Immunogen: E. coli-derived recombinant human Caspase-3 (18 kDa subunit) aa 29-175
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Protein A purified
Format B: liquid
Species Reactivity: Human
Entrez: 836
UniProt: P42574
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$319.00Add to Cart

Caspase-3 (Cysteine-aspartic acid protease 3/Casp3; also Yama, apopain and CPP32) is a 29 kDa heterodimer that belongs to the peptidase C14A family of enzymes. It is widely expressed, and considered to be the major executioner caspase in the apoptotic cascade. Human procaspase-3 is a 32 kDa, 277 amino acid (aa) protein and is normally an inactive homodimer. Following cell stress/activation, procaspase-3 undergoes proteolysis to generate an N-terminal 148 aa p17/17 kDa subunit (aa 29-175), plus a 102 aa C-terminal p12/12 kDa subunit. These subunits noncovalently heterodimerize, and associate with another p17/p12 heterodimer to form an active enzyme.

Images
Western Blot

Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 μM staurosporine (STS) for 3 hours. PVDF membrane was probed with 1 µg/mL of Human Caspase‑3 Monoclonal Antibody, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. Specific bands were detected for precursor Caspase‑3 and the p18 subunit at approximately 36 and 18 kDa (as indicated).

Western Blot

Western blot shows lysates of Jurkat human acute T cell leukemia cell line untreated (-) or treated (+) with 1 μM staurosporine (STS) for 3 hours. PVDF membrane was probed with 1 µg/mL of Human Caspase‑3 Monoclonal Antibody, followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. Specific bands were detected for precursor Caspase‑3 and the p18 subunit at approximately 36 and 18 kDa (as indicated).