Product Details
Catalog Number: MO15147
Applications: WB
Type: Mouse IgG
Dilutions: Western Blot: 2µg/mL
Immunogen: E. coli-derived recombinant human IL 10 Ser19-Asn178
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles
Shipping: Frozen (Polar Packs)
Format A: Protein A purified
Format B: liquid
Species Reactivity: Human
Entrez: 3586
UniProt: P22301
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$275.00Add to Cart

Interleukin 10, also known as IL-10, is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II Ags, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

Images

Detection of Human IL 10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL 10 Monoclonal Antibody (Catalog # MO15147) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for IL 10 at approximately 16 kDa (as indicated).

Detection of Human IL 10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human IL 10 Monoclonal Antibody (Catalog # MO15147) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody. A specific band was detected for IL 10 at approximately 16 kDa (as indicated).