Product Details
Catalog Number: MO15150
Applications: ICC, WB
Type: Mouse IgG
Dilutions: Western Blot: 0.5 µg/mL Immunocytochemistry: 8-25 µg/mL
Immunogen: E. coli-derived recombinant human Afadin/AF-6 Asn217-Pro392
Storage: Antibody can be stored for 6 months -20° C to -70° C. The antibody can be stored at 2° - 8° C for 1 month. Avoid repeated freeze-thaw cycles.
Shipping: Shipped on Ice Packs
Species Reactivity: Human
Entrez: 4301
UniProt: P55196
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$389.00Add to Cart

Afadin, also known as MLLT-4 (Myeloid/Lymphoid or mixed-lineage Leukemia Translocated to Ch 4) and AF-6,  is a 200-210 kDa nectin and F-actin-binding intracellular protein. It is ubiquitously expressed, and serves as an intermediary that links nectin with the actin cytoskeleton in tight and adherens junctions (AJs). Afadin is involved in the nectin-mediated recruitment of catenins to zonule adherens complexes, and in the recruitment of ZO-1 to the apical side of AJs for the purpose of forming tight junctions.

Images
Immunocytochemistry

Afadin/AF-6 was detected in immersion fixed BEAS2B human bronchial epithelial cell line using Mouse Anti-Human Afadin/AF-6 Monoclonal Antibody (Catalog #MO15150) at 25 µg/mL for 3 hours at room temperature

Immunocytochemistry

Afadin/AF-6 was detected in immersion fixed BEAS2B human bronchial epithelial cell line using Mouse Anti-Human Afadin/AF-6 Monoclonal Antibody (Catalog #MO15150) at 25 µg/mL for 3 hours at room temperature

Western Blot

Western blot shows lysates of IMR‑32 human neuroblastoma cell line and SK‑OV‑3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Afadin/AF-6 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody.

Western Blot

Western blot shows lysates of IMR‑32 human neuroblastoma cell line and SK‑OV‑3 human ovarian adenocarcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Afadin/AF-6 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody.