Product Details
Catalog Number: MO15163
Applications: ICC, WB
Type: Mouse IgG
Dilutions: Western Blot: 0.1 µg/mL Immunocytochemistry: 8-25 µg/mL
Immunogen: E. coli-derived recombinant human ATG12 Asp48-Ala187
Storage: Antibody can be stored for 6 months -20° C to -70° C. The antibody can be stored at 2° - 8° C for 1 month. Avoid repeated freeze-thaw cycles.
Shipping: Shipped on Ice Packs
Format A: liquid
Species Reactivity: Human, Mouse
Entrez: 9140
UniProt: O94817
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$389.00Add to Cart

Detects endogenous human, mouse and rat BNIP3L in Western blots. In Western blots, less than 10% cross-reactivity to recombinant human BNIP3L is observed. Bcl-2/adenovirus E1B 19 kDa protein-interacting protein 3-like (BNIP3L) also known as BNIP3 alpha and NIP3-like protein X (Nix), is a proapoptotic member of BNIP3 protein family. BNIP3L is a functional homolog of BNIP3 and both proteins contain a single Bcl-2 homology 3 (BH3) domain.

Images
Immunocytochemistry

ATG12 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line untreated (lower panel) or stimulated with LPS (upper panel) using Mouse Anti-Human/Mouse ATG12 Monoclonal Antibody at 15 µg/mL for 3 hours at room temperature.

Immunocytochemistry

ATG12 was detected in immersion fixed RAW 264.7 mouse monocyte/macrophage cell line untreated (lower panel) or stimulated with LPS (upper panel) using Mouse Anti-Human/Mouse ATG12 Monoclonal Antibody at 15 µg/mL for 3 hours at room temperature.

Western Blot

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HCT‑116 human colorectal carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane (left) was probed with 0.1 µg/mL of Mouse Anti-Human ATG12 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody.

Western Blot

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HCT‑116 human colorectal carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane (left) was probed with 0.1 µg/mL of Mouse Anti-Human ATG12 Monoclonal Antibody followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody.