Product Details
Catalog Number: MO15168
Applications: WB, IHC
Type: Rabbit IgG
Dilutions: Western Blot: 1 µg/mL Immunohistochemistry: 3-25 µg/mL
Immunogen: Synthetic peptide containing human LC3A peptide Accession # Q9H492
Storage: Antibody can be stored for 6 months -20° C to -70° C. The antibody can be stored at 2° - 8° C for 1 month. Avoid repeated freeze-thaw cycles.
Shipping: Shipped on Ice Packs
Format A: liquid
Species Reactivity: Human
Entrez: 84557
UniProt: Q9H492
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$399.00Add to Cart

Detects human LC3A in direct ELISA. Human Microtubule-associated Protein (MAP) Light Chain 3 (LC3) A is a121 amino acid (aa) protein with a predicted molecular weight of 14 kDa. It is a member of the LC3 subfamily of Autophagy-related 8 (Atg8) proteins (1). The LC3 subfamily also includes LC3B andLC3C. LC3 exhibits 100% aa sequence identity with its mouse and rat orthologs, and is orthologous to the yeast autophagy-related protein Atg8. Atg8 family members show structural similarity with Ubiquitin, but lack aa sequence similarity. LC3 was originally described as part is part of a complex that includes heavy and light chains comprising the MAP1 family of microtubule regulatory proteins (3). However, LC3 has gained attention for MAP1-independent functions in autophagy.

Images
Western Blot

Western blot shows lysates of A549 human lung carcinoma cell line and human brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human LC3A Monoclonal Antibody

Western Blot

Western blot shows lysates of A549 human lung carcinoma cell line and human brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human LC3A Monoclonal Antibody

Immunohistochemistry

LC3A was detected in immersion fixed paraffin-embedded sections of human brain (frontal cortex) using Rabbit Anti-Human LC3A Monoclonal Antibody at 3 µg/mL for 1 hour at room temperature.

Immunohistochemistry

LC3A was detected in immersion fixed paraffin-embedded sections of human brain (frontal cortex) using Rabbit Anti-Human LC3A Monoclonal Antibody at 3 µg/mL for 1 hour at room temperature.