Product Details
Catalog Number: MO20025
Applications: IHC
Type: Mouse IgG
Immunogen: Prokaryotic recombinant protein corresponding to the external domain of the human CD4 molecule. Clone: 1F6
Storage: Store at 4°C short term. Store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Supernatant
Format B: liquid
Species Reactivity: Human
Entrez: 920
UniProt: P01730
Downloads: Datasheet (pdf)
Downloads: SDS (pdf)
Product Sizes
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100 ul$155.00Add to Cart

CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. The CD4 antigen is expressed on a T cell subset (helper/inducer) representing 45 percent of peripheral blood lymphocytes and at a lower level on monocytes. Most cases of cutaneous T cell lymphoma, including mycosis fungoides, express the CD4 antigen and HTLV-1 associated adult T cell leukemia/lymphoma is also generally CD4 positive.

Note: the use of 1 percent or greater H2O2 to block endogenous peroxidase has a detrimental effect on the epitope recognized by clone 1F6. Therefore, it is recommended that endogenous peroxidase is blocked before retrieval with 0.5 percent H2O2 /methanol for 10 minutes, otherwise staining intensity may be reduced. 

Images
CD4 staining of  Human skin, mycosis fungoides. Note membrane staining of infiltrating T lymphocytes. Paraffin section

CD4 staining of Human skin, mycosis fungoides. Note membrane staining of infiltrating T lymphocytes. Paraffin section

CD4 staining of  Human skin, mycosis fungoides. Note membrane staining of infiltrating T lymphocytes. Paraffin section

CD4 staining of Human skin, mycosis fungoides. Note membrane staining of infiltrating T lymphocytes. Paraffin section