Product Details
Catalog Number: MO22103
Applications: ICC, WB, IHC
Type: Mouse IgG
Dilutions: ICC 1:1000; IHC 1:1000; WB 1:10,000
Immunogen: Native NF-H purified from pig spinal cord
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Primate, Chicken
Entrez: 4744
UniProt: P12036
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 ul$248.00Add to Cart

Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope, neurofilaments appear as 10nm diameter fibers of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.

This antibody recognizes phosphorylated NF-H KSP (lysine-serine-proline) type sequences. In some species, there is some cross-reactivity with the related phosphorylated KSP sequences found in the related neurofilament subunit NF-M. The antibody recognizes NF-H strongly in all mammals tested to date and also in chicken. It recognizes neurofilaments in frozen sections in tissue culture and in formalin fixed sections

Images
Mixed neuron/glial cultures stained with MO22103 (red) and rabbit GFAP antibody (green). Axonal profiles are stained in red, while astrocytic cells are revealed in green. Nuclei are revealed with a fluorescent DNA stain (blue)

Mixed neuron/glial cultures stained with MO22103 (red) and rabbit GFAP antibody (green). Axonal profiles are stained in red, while astrocytic cells are revealed in green. Nuclei are revealed with a fluorescent DNA stain (blue)

Mixed neuron/glial cultures stained with MO22103 (red) and rabbit GFAP antibody (green). Axonal profiles are stained in red, while astrocytic cells are revealed in green. Nuclei are revealed with a fluorescent DNA stain (blue)

Mixed neuron/glial cultures stained with MO22103 (red) and rabbit GFAP antibody (green). Axonal profiles are stained in red, while astrocytic cells are revealed in green. Nuclei are revealed with a fluorescent DNA stain (blue)