Product Details
Catalog Number: MO22139
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Immunogen: Full length recombinant human Aurora C protein expressed in and purified from E. coli.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Chicken, Bovine, Porcine, Equine
Entrez: 6790
UniProt: O14965
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$325.00Add to Cart

Aurora proteins are a family of proteins that play a key role in the regulation of cell division. MO22139 was raised against full-length recombinant human aurora C expressed in and purified from E. coli, it has been shown to react with all three aurora kinase (blot image). 

Images
Western blot analysis of Aurora A/B/C (MO22139) kinase in HeLa cells

Left: Western blot analysis of Aurora A/B/C kinase in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with Aurora A/B/C. The antibody monoclonal binds strongly to bands at about 46 kDa and 38kDa, corresponding to Aurora A and Aurora B. Also, it recognizes a weak band at 35 kDa which is Aurora C. Right: Blot of recombinant full length human Aurora A, B and C was probed with MO22139. This antibody reacts strongly with all three Aurora kinases proteins.

Western blot analysis of Aurora A/B/C (MO22139) kinase in HeLa cells

Left: Western blot analysis of Aurora A/B/C kinase in HeLa cells. Blot of HeLa cells treated with 100ng/ml nocodazole for 18 hours was probed with Aurora A/B/C. The antibody monoclonal binds strongly to bands at about 46 kDa and 38kDa, corresponding to Aurora A and Aurora B. Also, it recognizes a weak band at 35 kDa which is Aurora C. Right: Blot of recombinant full length human Aurora A, B and C was probed with MO22139. This antibody reacts strongly with all three Aurora kinases proteins.

HeLa cell cultures were stained with Aurora A/B/C kinase antibody (green).

HeLa cell cultures were stained with Aurora A/B/C kinase antibody (green). In this image, strong staining in spindle poles was seen in cells at anaphase. This antibody also stains the midbodies between the two daughter cells. Counterstained is our chicken polyclonal antibody against Vimentin, (red, CH22108). Blue is a DNA stain.

HeLa cell cultures were stained with Aurora A/B/C kinase antibody (green).

HeLa cell cultures were stained with Aurora A/B/C kinase antibody (green). In this image, strong staining in spindle poles was seen in cells at anaphase. This antibody also stains the midbodies between the two daughter cells. Counterstained is our chicken polyclonal antibody against Vimentin, (red, CH22108). Blue is a DNA stain.