Product Details
Catalog Number: MO22146
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Immunogen: Full-length recombinant human protein. HGNC name for this protein is CALB1.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Chicken, Porcine
Entrez: 793
UniProt: P05937
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$325.00Add to Cart

Calbindin, also known as calbindin 1 or calbindin-D28k is a member of the large superfamily of cytoplasmic Ca2+ binding proteins. Calbindin-1 belongs to the subclass of these containing the "EF hand" Ca2+ binding motif originally characterized in parvalbumin. Calbindin is expressed in mammalian brain, intestine, kidney and pancreas. In the brain it is localized in certain classes of neurons, and antibodies to it are useful for identifying specific neuronal cell types. It is particularly concentrated in the dendrites and perikarya of cerebellar Purkinje cells, but is also found in many GABAergic interneurons in the cortex.

The function of calbindin-1 appears to be primarily buffering the Ca2+ level in cells. The affinity of calbindin for Ca2+ is low at the typical resting cytoplasmic Ca2+ level of around 100 nM, and the protein only binds Ca2+ significantly when levels increase greatly. Accordingly, it is widely thought that the primary function of this protein is to act as a Ca2+ buffer. Buffering Ca2+ is important, as uncontrolled increases in the level of this cation can lead to both apoptosis due to Ca2+stimulated release of proteins from mitochondria and necrosis due to the activation of Ca2+dependent proteases.

Images

Rat brain cerebellum (Left) and cortex (Right) sections (45 μM; fixed by transcardial perfusion with 4%paraformaldehyde) were co-stained with our Calbindin (MO22146) (red) and anti-MeCP2 rabbit antibody (RA22123) (green).

Rat brain cerebellum (Left) and cortex (Right) sections (45 μM; fixed by transcardial perfusion with 4%paraformaldehyde) were co-stained with our Calbindin (MO22146) (red) and anti-MeCP2 rabbit antibody (RA22123) (green).