Product Details
Catalog Number: MO22189
Applications: ICC, WB, IHC, IF
Type: Mouse IgG
Dilutions: Immunofluorescent: 1:1,000 Immunocytochemistry: 1:1,000 Immunohistochemistry: 1:1,000 Western Blot: 1:5,000-10,000
Immunogen: Purified myelin basic protein isolated from bovine brain, epitope is in peptide TPPPSQGKG, amino acids 125-133 of the human 21.5kDa sequence
Storage: Store at 4°C short term. Store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine, Equine
Entrez: 4155
UniProt: P02686
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

Myelin Basic Protein (MBP) is one of the major proteins of the myelin sheath surrounding axons in the nervous system. Since it is of relatively low molecular weight and high abundance the protein sequence was determined from purified protein over 30 years ago. The protein is made by oligodendrocytes in the central and nervous system, so antibodies to MBP are good markers of this cell type. In the peripheral nervous system MBP is expressed by myelinating Schwann cells so this antibody can be used to identify these cells in culture or sectioned materials. In the central nervous system four different forms of the protein made by alternate transcription from a single gene, the protein products having molecular weights of 21.5, 20.5, 18.5, and 17.2kDa in humans. The single gene of rodents also produces 4 different proteins but the splicing mechanism is different producing four forms of slightly different sizes, 21.5, 18.5, 17 and 14kDa. Some interest has focused on MBP as a potentially significant auto-antigen involved in mouse models of multiple sclerosis and in human patients. Detection of MBP released into blood and CSF has some potential as a surrogate biomarker of demyelination and axonal loss in MS and other relevant damage and disease states.

Images
Immunofluorescent

Immunofluorescent analysis of rat hippocampal section stained with mouse mAb to myelin basic protein (MBP), MO22189, dilution 1:5,000 in green, and costained with rabbit pAb to neurofilament NF-H dilution 1:2,000, in red. The MBP antibody stains oligodendrocyte cell bodies and the myelin sheathes around axons, while the NF-H antibody labels the axons themselves.

Immunofluorescent

Immunofluorescent analysis of rat hippocampal section stained with mouse mAb to myelin basic protein (MBP), MO22189, dilution 1:5,000 in green, and costained with rabbit pAb to neurofilament NF-H dilution 1:2,000, in red. The MBP antibody stains oligodendrocyte cell bodies and the myelin sheathes around axons, while the NF-H antibody labels the axons themselves.

Western Blot

Western blot analysis of different tissue lysates using mouse mAb to MBP dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] rat sciatic nerve, [5] mouse brain, [6] mouse spinal cord. Multiple bands at 14kDa, 17kDa, 18.5kDa to 21.5kDa are the alternate transcripts of MBP. Other bands are proteolytic fragments of the MBP protein.

Western Blot

Western blot analysis of different tissue lysates using mouse mAb to MBP dilution 1:20,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] rat sciatic nerve, [5] mouse brain, [6] mouse spinal cord. Multiple bands at 14kDa, 17kDa, 18.5kDa to 21.5kDa are the alternate transcripts of MBP. Other bands are proteolytic fragments of the MBP protein.