Product Details
Catalog Number: MO22199
Applications: ICC, WB, IF
Type: Mouse IgG
Dilutions: Immunofluorescent: 1:1,000 Immunocytochemistry: 1:1,000 Western Blot: 1:1,000
Immunogen: C-terminal peptide of human ?-synuclein EPEGESYEDPPQEEYQEYEPEA coupled to KLH
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Bovine, Porcine
Entrez: 6622
UniProt: P37840
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

β-synuclein is a member of the synuclein protein family, the other two members being α and γ-synuclein, each protein being coded for by a distinct but related gene. α-synuclein was originally isolated as a major synaptic vesicle associated protein from the electric organ of the fish Torpedo, and direct homologues of α-synuclein are found in all vertebrates. Later work connected α-synuclein expression with several human brain pathologies, so that it is a major component of the Lewy bodies of Parkinson’s disease. β-synuclein was isolated as a component of normal and diseased human brain as a protein clearly related to but distinct from α-synuclein. The human β-synuclein molecule is 134 amino acids in size compared to 140 amino acids for α-synuclein, and the N-terminal halves of the two molecules are virtually identical while the C-terminal regions is more variable.

Images
Immunofluorescence

Immunofluorescent analysis of mouse hippocampus section stained with mouse mAb to β-synuclein, MO22190, dilution 1:500 in green, and costained with rabbit peptide pAb to neurofilament NF-L, dilution 1:5,000, in red. The blue is Hoechst staining of nuclear DNA. The β-synuclein antibody detects protein concentrated in synaptic regions, while the NF-L antibody labels dendrites and axons of neuronal cells.

Immunofluorescence

Immunofluorescent analysis of mouse hippocampus section stained with mouse mAb to β-synuclein, MO22190, dilution 1:500 in green, and costained with rabbit peptide pAb to neurofilament NF-L, dilution 1:5,000, in red. The blue is Hoechst staining of nuclear DNA. The β-synuclein antibody detects protein concentrated in synaptic regions, while the NF-L antibody labels dendrites and axons of neuronal cells.

Western Blot

Western blot analysis of tissue lysates using mAb to α-synuclein, dilution 1:1,000 in green. Lane [1] protein standard in red, [2] whole rat brain lysate, [3] rat spinal cord lysate. The strong band at about 15kDa corresponds to α-synuclein protein.

Western Blot

Western blot analysis of tissue lysates using mAb to α-synuclein, dilution 1:1,000 in green. Lane [1] protein standard in red, [2] whole rat brain lysate, [3] rat spinal cord lysate. The strong band at about 15kDa corresponds to α-synuclein protein.