Product Details
Catalog Number: MO22200
Applications: ICC, WB, IF
Type: Mouse IgG
Dilutions: Immunofluorescent: 1:1,000-2,000 Immunocytochemistry: 1:1,000-2,000 Western Blot: 1:2,000-5,000
Immunogen: Full length human annexin A5 expressed in and purified from E. coli
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Primate
Entrez: 308
UniProt: P08758
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$275.00Add to Cart

The annexins are a large family of related proteins which share the property of binding to phophospholipid containing membranes in a Calcium dependent manner. Different members of the family were discovered by different laboratories and as a result the various members have many alternate names, such as lipocortin, calpactin, calelectrin and very many others. The widely used current nomenclature is now based on a letter to indicate membership in a particular one of several annexin sub-families and a number for individual gene products, hence the name annexin A5. The annexin family is defined by a compact disc structure formed from 16 closely packed α-helices which co-ordinate multiple calcium ions with phospholipid containing membranes. This domain is defined by 4 imperfect repeats of a ~77 amino acid sequence, each repeat forming 4 α-helices. Annexin A5 is expressed widely in tissues and has been used as a marker of apoptosis, as apoptotic cells may express binding sites for this protein on their cell surface. The protein binds to phosphatidylserine, a membrane lipid normally not found on the external surface of cells which becomes expressed on the cell surface during apoptosis. As a result fluorescent annexin A5 or annexin A5 antibody can be use to isolate apoptotic cells by fluorescence activated cell sorting.

Images
Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with mouse mAb to annexin A5, MO22200, dilution 1:1,000 in green, and costained with chicken pAb to vimentin dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA.

Immunofluorescence

Immunofluorescent analysis of HeLa cells stained with mouse mAb to annexin A5, MO22200, dilution 1:1,000 in green, and costained with chicken pAb to vimentin dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA.

Western Blot

Western blot analysis of different cell lines lysates using mouse mAB to annexin A5 dilution 1:2,000 in green: [1] protein standard (red), [2] mouse NIH-3T3, [3] rat C6, [4] human HeLa, [5] human HEK293, [6] canine A72, [7] equine NBL6, and [8] African green monkey COS1 cells.

Western Blot

Western blot analysis of different cell lines lysates using mouse mAB to annexin A5 dilution 1:2,000 in green: [1] protein standard (red), [2] mouse NIH-3T3, [3] rat C6, [4] human HeLa, [5] human HEK293, [6] canine A72, [7] equine NBL6, and [8] African green monkey COS1 cells.