Product Details
Catalog Number: MT27000
Product Sizes
SizeList PricePriceCart
<1000 ug Nucleic Acids$999.00Add to Cart
<200 ug Nucleic Acids$249.00Add to Cart

MATra-A contains magnetic nanoparticles (MagTag™) which can be loaded with the nucleic acid of interest. Exploiting magnetic force the full nucleic acid dose is then rapidly drawn towards and delivered into the target cells leading to efficient transfection. MATra-A can be used for MATra-A Reagent adherent cells.

Comprehensive Protocol for All MATra-Three different procedures according to different initial situations can be followed to apply MATra:

  • MATra for adherent cells Magnet Assisted Transfection for adherent cells. The nucleic acid has to be combined with MATra-A (plasmid DNA) or MATRa-si (siRNA) Reagent and MATra can be performed directly .
  • MATra for suspension cells-Magnet Assisted Transfection for suspension cells. Suspension cells have to be made adherent first by incubating them with the magnetic reagent MATRa-S Immobilizer or by using polylysine plates. Then MATra-A or MATra-si Reagent loaded with the nucleic acid can be applied and MATra can be performed.
  • MATra Lipofection for adherent and suspension cells-Transfection with common lipid based (e.g. i-Fect™, pn-Fect™, IBA-Fect™, Lipofectamine or Fugene) or polycationic reagents (e.g. ExGen500 or Superfect) can be enhanced by magnetic assistance. In this case, the nucleic acid is combined with MA Lipofection Enhancer in the presence of the common lipofection reagent. In addition, the MA Lipofection Enhancer can be used for viral transfection.

Images

Primary cortical neurons from mice embryonic day 15.5 (E15.5) were grown on poly-L-lysine coated coverslips at a density of 800.000 cells/well in a 24-well plate. The neurons were transfected after 1 day in vitro (DIV 1) with pCX-EGFP-N1 plasmid. Transfection was carried out as recommended by the manufacturer (0.6 µg DNA, 0.6 µL Matra-A reagent). Cells were fixed 24 h later (DIV 2) and GFP fluorescence was visualized using a confocal laser scanning microscope.

Primary cortical neurons from mice embryonic day 15.5 (E15.5) were grown on poly-L-lysine coated coverslips at a density of 800.000 cells/well in a 24-well plate. The neurons were transfected after 1 day in vitro (DIV 1) with pCX-EGFP-N1 plasmid. Transfection was carried out as recommended by the manufacturer (0.6 µg DNA, 0.6 µL Matra-A reagent). Cells were fixed 24 h later (DIV 2) and GFP fluorescence was visualized using a confocal laser scanning microscope.