Suspension cells have to be made adherent first by incubating them with the reagent MATra-S Immobilizer which contains magnetic nanoparticles (MagTag™).
Image: Universal magnet plate (8 x 13 cm) with collection of different culture plates and MATra reagents.
Comprehensive Protocol for All MATra-Three different procedures according to different initial situations can be followed to apply MATra:
MATra Lipofection for adherent and suspension cells-Transfection with common lipid based (e.g. i-Fect ™, pn-Fect™ , IBAfect , Lipofectamine or Fugene) or polycationic reagents (e.g. ExGen500 or Superfect) can be enhanced by magnetic assistance. In this case, the nucleic acid is combined with MA Lipofection Enhancer in the presence of the common lipofection reagent. In addition, the MA Lipofection Enhancer can be used for viral transfection.
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