Product Details
Catalog Number: PC35115
Applications: Cell Assays
Type: Primary Cells
Storage: Store for up at 7 days at 4°C-8°C.
Shipping: Refrigerated (Polar Packs)
Species Reactivity: Rat
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
10 DRGs$705.00Add to Cart

Primary Rat DRGs are live neurons isolated from micro-surgically dissected regions of day 18 embryonic Sprague/Dawley rat brain. These cells are prepared fresh each week and shipped in a nutrient rich medium that keeps the cells alive for up to 14 days under refrigeration.

Please note: It is important to review Protocol/Datasheet prior to ordering. There is a unique step for making the dissociation enzyme solution. Do not hesitate to call or e-mail me (612-801-1007 or pshuster@neuromics.com) should you have questions.

Testimonial: "Thanks for following up, the DRGs worked great and we were able to get excellent data from them. Thanks so much for working with us." Adam Ross, Dr. Chengji Zhou Lab, UC Davis

References: A dissection and Tissue Culture Manual of the Nervous System (1989). A. Shahar, J.D. Vellis, A. Vernadakis, B. Haber (Eds.), Dissociated Spinal Cord - Dorsal Root Ganglion Cultures on Plastic Tissue Culture Dishes and Glass Coverslips and Wells (pp.219-222). Wiley-Liss, Inc. J.L. Werth,
S.A. Thayer (1994) Mitrochondria Buffer Physiological Calcium Loads in Cultured Rat Dorsal Root Ganglion Neurons, The Journal of Neuroscience, 14(1), 348-356

Images

DRGs cultured on Calf Skin Collagen.

DRGs cultured on Calf Skin Collagen.

DRGs cultured on Poly-D-Lysine

DRGs cultured on Poly-D-Lysine

Above image of rat embryonic dorsal root ganglia. DRGs were cultured on coverslips, and then infected with herpes simplex virus type I. The virus has an mCherry fluorescent protein tag on the capsid shell; which can be visualized in the photo. Data courtesy of Austin

Above image of rat embryonic dorsal root ganglia. DRGs were cultured on coverslips, and then infected with herpes simplex virus type I. The virus has an mCherry fluorescent protein tag on the capsid shell; which can be visualized in the photo. Data courtesy of Austin