Product Details
Catalog Number: PR15001CF
Applications: Adherent Cell Assays
Type: Protein
Storage: Store at 4°C short term. Store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Ambient
Format A: Protein A purified
Format B: liquid
Species Reactivity: Bovine
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
1 mg$125.00Add to Cart
5 mg$315.00Add to Cart

Fibronectin purified from bovine plasma by affinity chromatography on gelatin-Sepharose.

Fibronectin is a high-molecular weight (~440kDa) extracellular matrix glycoprotein that binds to membrane-spanning receptor proteins called integrins.[1] In addition to integrins, fibronectin also binds extracellular matrix components such as collagen, fibrin and heparan sulfate proteoglycans (e.g. syndecans). Fibronectin exists as a dimer, consisting of two nearly identical monomers linked by a pair of disulfide bonds. The fibronectin protein is produced from a single gene, but alternative splicing of its pre-mRNA leads to the creation of several isoforms.

Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. In this application, the recommended concentration for this effect is typically 1 - 5 μg/cm2. Fibronectin can also be added in the media to support cell spreading at a concentration of 0.5 - 50 μg/mL. Optimal concentration depends on cell type as well as the application or research objectives.

 Fibronectin plays a major role in cell adhesion, growth, migration and differentiation, and it is important for processes such as wound healing and embryonic development. Altered fibronectin expression, degradation, and organization has been associated with a number of pathologies, including cancer and fibrosis.

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