Golgi-associated ATPase Enhancer of 16 kDa (GATE-16), also known as Apg8p2 and GABARAPL2, is a 117 amino acid (aa) polypeptide and a member of the Autophagy-related 8 (Atg8) family of proteins. GATE-16/Apg8p2 is best known for its role in autophagy. GATE-16/Apg8p2 covalently attaches to phosphatidylethanolamine (PE) the phagophore (autophagosome precursor) membrane using a Ubiquitin-like conjugation system that includes Ubiquitin-activating (E1)-, Ubiquitin-conjugating (E2)-, and Ubiquitin Ligase (E3)-like enzymes. Here it is involved in the later stages of autophagosome formation. It may also be involved in cargo recruitment to autophagosomes.
His6-GATE-16/Apg8p2 is modified with rhodamine red via primary amine coupling. This results in rhodamine coupled GATE-16/Apg8p2 species with modified lysines as well as the N-terminus. Detection of rhodamine-GATE-16/Apg8p2 occurs with higher efficiency and sensitivity than with antibodies. This protein contains an N-terminal His6 tag.
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