Product Details
Catalog Number: PR15112CF
Type: Protein
Storage: This product can be stored for up to 1 month at 2°C to 8°C under sterile condition after reconstitution, for up to 3 months at -20°C to -70°C under sterile condition after reconstitution, or for up to 12 months at -20°C to -70°C, as supplied. Avoid freeze-thaw cycles.
Shipping: Ambient
Format B: lyophilized
Species Reactivity: Human
Entrez: 1950
UniProt: P01133
Downloads: Datasheet (pdf)
Product Sizes
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200 ug$269.00Add to Cart

Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGF-alpha, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparin‑binding EGF‑like growth factor (HB‑EGF), epigen, and the neuregulins (NRG)-1 through 6. EGF is present in various body fluids, including blood, milk, urine, saliva, seminal fluid, pancreatic juice, cerebrospinal fluid, and amniotic fluid. Four ErbB (HER) family receptor tyrosine kinases including EGFR/ErbB1, ErbB2, ErbB3 and ErbB4, mediate responses to EGF family members. EGF binds ErbB1 and depending on the context, induces the formation of homodimers or heterodimers containing ErbB2. Dimerization results in autophosphorylation of the receptor at specific tyrosine residues to create docking sites for a variety of signaling molecules. Biological activities ascribed to EGF include epithelial development, angiogenesis, inhibition of gastric acid secretion, fibroblast proliferation, and colony formation of epidermal cells in culture.

Images
Bioactivity

GMP-grade Recombinant Human EGF stimulates proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is 20‑100 pg/mL.

Bioactivity

GMP-grade Recombinant Human EGF stimulates proliferation of the Balb/3T3 mouse embryonic fibroblast cell line. The ED50 for this effect is 20‑100 pg/mL.

SDS-PAGE

1 µg/lane of GMP-grade Recombinant Human EGF was resolved by SDS-PAGE and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.

SDS-PAGE

1 µg/lane of GMP-grade Recombinant Human EGF was resolved by SDS-PAGE and visualized by silver staining under reducing (R) conditions, showing a single band at 6 kDa.

Mass Spectrometry

MALDI‑TOF analysis of GMP‑grade Recombinant Human EGF. The major peak corresponds to the calculated molecular mass, 6353 Da. The minor peak at 6549 Da is a matrix-associated artifact of the MALDI-TOF.

Mass Spectrometry

MALDI‑TOF analysis of GMP‑grade Recombinant Human EGF. The major peak corresponds to the calculated molecular mass, 6353 Da. The minor peak at 6549 Da is a matrix-associated artifact of the MALDI-TOF.