Product Details
Catalog Number: PR15114CF
Type: Protein
Storage: This product can be stored for up to 1 month at 2°C to 8°C under sterile condition after reconstitution, for up to 3 months at -20°C to -70°C under sterile condition after reconstitution, or for up to 12 months at -20°C to -70°C, as supplied. Avoid freeze-thaw cycles.
Shipping: Ambient
Format B: lyophilized
Species Reactivity: Human
Entrez: 3565
UniProt: P05112
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
10 ug$389.00Add to Cart
50 ug$1,199.00Add to Cart

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses. It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha-helix structure. IL-4 exerts its effects through two receptor complexes. The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, 7, 9, 15, and 21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL‑13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils. It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells. IL-4 plays a dominant role in the development of allergic inflammation and asthma.

Images
Bioactivity

GMP-grade Recombinant Human IL‑4 stimulates proliferation of TF‑1 human erythroleukemic cells. The ED50 is 0.05‑0.2 ng/mL.

Bioactivity

GMP-grade Recombinant Human IL‑4 stimulates proliferation of TF‑1 human erythroleukemic cells. The ED50 is 0.05‑0.2 ng/mL.

SDS-PAGE

1 μg/lane of GMP-grade Recombinant Human IL-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.

SDS-PAGE

1 μg/lane of GMP-grade Recombinant Human IL-4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 14 kDa.

Mass Spectrometry

MALDI-TOF analysis of GMP-grade Recombinant Human IL-4. The major peak at 15083 Da corresponds to the calculated molecular mass, 15094 Da. The minor peak at 15297 Da is a matrix-associated artifact of the MALDI-TOF.

Mass Spectrometry

MALDI-TOF analysis of GMP-grade Recombinant Human IL-4. The major peak at 15083 Da corresponds to the calculated molecular mass, 15094 Da. The minor peak at 15297 Da is a matrix-associated artifact of the MALDI-TOF.