Product Details
Catalog Number: PR15115CF
Type: Protein
Storage: This product can be stored for up to 1 month at 2°C to 8°C under sterile condition after reconstitution, for up to 3 months at -20°C to -70°C under sterile condition after reconstitution, or for up to 12 months at -20°C to -70°C, as supplied. Avoid freeze-thaw cycles.
Shipping: Ambient
Format B: lyophilized
Species Reactivity: Human
Entrez: 3574
UniProt: P13232
Downloads: Datasheet (pdf)
Product Sizes
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25 ug$1,259.00Add to Cart

IL-7 (interleukin-7) is a 25 kDa cytokine of the hemopoietin family that plays important roles in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by a wide variety of cells in primary and secondary lymphoid tissues, including stromal epithelial cells of the thymus, bone marrow, and intestines. Circulating IL-7 is limiting in healthy animals, but increases during lymphopenia. IL-7 signals through a complex of the IL-7 Receptor alpha subunit (IL-7 R alpha, also known as CD127) with the common gamma chain (gamma c). The gamma c is also a subunit of the receptors for IL-2, 4, 9, 15, and 21. IL-7 R alpha is expressed on double negative (CD4-CD8-) and CD4+ or CD8+ single positive naïve and memory T cells, but undergoes IL-7-mediated down‑regulation and shedding during antigen-driven T cell proliferation, and is absent on regulatory T cells. IL-7 contributes to the maintenance of all naïve and memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl-2.

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Bioactivity

GMP-grade Recombinant Human IL-7 stimulates proliferation of PHA-activated human peripheral blood lymphocytes. The ED50 for this effect is 0.1-0.5 ng/mL.

Bioactivity

GMP-grade Recombinant Human IL-7 stimulates proliferation of PHA-activated human peripheral blood lymphocytes. The ED50 for this effect is 0.1-0.5 ng/mL.

Mass Spectrometry

MALDI-TOF analysis of GMP-grade Recombinant Human IL-7. The major peak corresponds to the calculated molecular mass, 17518 Da. The minor peak at 17711 Da is a matrix-associated artifact of the MALDI-TOF.

Mass Spectrometry

MALDI-TOF analysis of GMP-grade Recombinant Human IL-7. The major peak corresponds to the calculated molecular mass, 17518 Da. The minor peak at 17711 Da is a matrix-associated artifact of the MALDI-TOF.

SDS-PAGE

1 μg/lane of GMP-grade Recombinant Human IL-7 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.

SDS-PAGE

1 μg/lane of GMP-grade Recombinant Human IL-7 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.