Product Details
Catalog Number: PR15121CF
Type: Protein
Storage: This product can be stored for up to 1 month at 2°C to 8°C under sterile condition after reconstitution, for up to 3 months at -20°C to -70°C under sterile condition after reconstitution, or for up to 12 months at -20°C to -70°C, as supplied. Avoid freeze-thaw cycles.
Shipping: Ambient
Format B: lyophilized
Species Reactivity: Human
Entrez: 89780
UniProt: P56704
Downloads: Datasheet (pdf)
Product Sizes
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10 ug$459.00Add to Cart

Wnt-3a is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins important for normal developmental processes. Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta-catenin levels. During embryonic development, Wnt-3a is necessary for proper development of the hippocampus, anterior-posterior patterning, somite development, and tailbud formation. Wnt-3a also promotes self-renewal of hematopoietic stem cells, neural stem cells, and embryonic stem cells.

Images
Bioactivity 1

GMP-grade Recombinant Human Wnt-3a induces alkaline phosphatase production by the MC3T3‑E1 mouse preosteoblast cell line. The ED50 for this effect is 5-25 ng/mL.

Bioactivity 1

GMP-grade Recombinant Human Wnt-3a induces alkaline phosphatase production by the MC3T3‑E1 mouse preosteoblast cell line. The ED50 for this effect is 5-25 ng/mL.

Bioactivity 2

GMP-grade Recombinant Human Wnt-3a induces beta -catenin-responsive transcriptional activation in a Topflash Luciferase assay using the HEK293T human embryonic kidney cell line. The ED50 for this effect is 500 ng/mL.

Bioactivity 2

GMP-grade Recombinant Human Wnt-3a induces beta -catenin-responsive transcriptional activation in a Topflash Luciferase assay using the HEK293T human embryonic kidney cell line. The ED50 for this effect is 500 ng/mL.