Product Details
Catalog Number: PR15126CF
Type: Protein
Storage: This product can be stored for up to 1 month at 2°C to 8°C under sterile condition after reconstitution, for up to 3 months at -20°C to -70°C under sterile condition after reconstitution, or for up to 12 months at -20°C to -70°C, as supplied. Avoid freeze-thaw cycles.
Shipping: Ambient
Format B: lyophilized
Species Reactivity: Human
Entrez: 7124
UniProt: P01375
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$1,109.00Add to Cart

Tumor necrosis factor alpha (TNF-alpha), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I.

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Bioactivity

GMP-grade Recombinant Human TNF-alpha induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 is 25-100 pg/mL.

Bioactivity

GMP-grade Recombinant Human TNF-alpha induces cytotoxicity in the L-929 mouse fibroblast cell line in the presence of the metabolic inhibitor actinomycin D. The ED50 is 25-100 pg/mL.

SDS-PAGE

1 μg/lane of GMP-grade Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a single band at 17 kDa.

SDS-PAGE

1 μg/lane of GMP-grade Recombinant Human TNF-alpha was resolved by SDS-PAGE with silver staining, under reducing (R) conditions, showing a single band at 17 kDa.

Mass Spectrometry

MALDI-TOF analysis of GMP-grade Recombinant Human TNF-alpha. The major peak at 17348 corresponds to the calculated molecular mass without an N-terminal Met, 17353 Da. The minor peak at 17480 corresponds to the calculated molecular mass with an N-terminal Met, 17484 Da. The peak at 17565 is a matrix-associated artifact of the MALDI-TOF.

Mass Spectrometry

MALDI-TOF analysis of GMP-grade Recombinant Human TNF-alpha. The major peak at 17348 corresponds to the calculated molecular mass without an N-terminal Met, 17353 Da. The minor peak at 17480 corresponds to the calculated molecular mass with an N-terminal Met, 17484 Da. The peak at 17565 is a matrix-associated artifact of the MALDI-TOF.