Product Details
Catalog Number: RA15043
Applications: ICC, WB, IHC
Type: Rabbit IgG
Immunogen: KLH coupled synthetic peptide CADLREDPDRQDHHPGSGAQ. The sequence corresponds to the carboxyl terminus of Ubiquitin+1.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human
Entrez: 7314
UniProt: P0CG47
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$365.00Add to Cart

Ubiquitin is a highly conserved protein that is required for ATP-dependent, nonlysosomal intracellular protein degradation of abnormal proteins and normal proteins with a rapid turnover. Ubiquitin is covalently bound to proteins to be degraded, and presumably labels these proteins for degradation.

Ubiquitin+1 has a carboxyl terminal amino acid sequence that differs from normal ubiquitin due to a frame shift mutation in the mRNA. This ubiquitin+1 form has been noticed in patients with Alzheimer's and Down's syndrome.

Images

Immunoblots of 2 ng recombinant human Ubiquitin and 2, 1, and 0.5 ng recombinant human Ubiquitin+1. Samples were electrophoresed on 15% gels and immunoblotting was with 1.0 μg/mL anti-Ubiquitin+1. A one minute exposure is shown.

Immunoblots of 2 ng recombinant human Ubiquitin and 2, 1, and 0.5 ng recombinant human Ubiquitin+1. Samples were electrophoresed on 15% gels and immunoblotting was with 1.0 μg/mL anti-Ubiquitin+1. A one minute exposure is shown.