Product Details
Catalog Number: RA15053
Applications: WB, IHC
Type: Rabbit IgG
Immunogen: Purified, E. coli-derived recombinant human p38 alpha mitogen-activated protein kinase (MAPK14) alpha (GenBank accession # Q16539).
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: lyophilized
Species Reactivity: Human, Mouse, Rat
Entrez: 1432
UniProt: Q16539
Downloads: Datasheet (pdf)
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100 ug$365.00Add to Cart

The p38 Mitogen-activated Protein Kinases (MAPKs) are a family of four related Ser/Thr kinases activated by proinflammatory cytokines and environmental stresses.

The most frequently analyzed family member, p38 alpha, also known as SAPK2a and MAPK14, was initially purified as a kinase critical to the signaling cascade linking IL-1 to MAPKAPK-2 and the small heat shock protein HSP27. Ubiquitously expressed, p38 alpha is dually phosphorylated by MKK3 and MKK6 at Thr180 and Tyr182. Once activated, p38 alpha phosphorylates a number of targets, including the cytoplasmic kinases MNK 4 and PRAK5 and the nuclear transcription factors ATF2 1 and STAT1. Several promising compounds that inhibit p38 alpha are being investigated as potential therapies for arthritic and inflammatory diseases.

Images

Detection of p38α by western blotting. Human HeLa cells, mouse NIH/3T3 cells, and rat PC12 cells were solubilized in SDS sample buffer and extract from 2 x 105cells was loaded in each lane. Extracts were run on a 12% polyacrylamide gel, transferred to Immobilon membrane, and immunoblotted with 0.25 μg/mL rabbit anti-p38α, as described in Datasheet. A 5 second exposure to film is shown.

Detection of p38α by western blotting. Human HeLa cells, mouse NIH/3T3 cells, and rat PC12 cells were solubilized in SDS sample buffer and extract from 2 x 105cells was loaded in each lane. Extracts were run on a 12% polyacrylamide gel, transferred to Immobilon membrane, and immunoblotted with 0.25 μg/mL rabbit anti-p38α, as described in Datasheet. A 5 second exposure to film is shown.

Orexin B and p38 alpha were detected in frozen sections of mouse brainstem using mouse anti-human Orexin B monoclonal antibody (red) and rabbit anti-human/mouse/rat p38 alpha affinity-purified polyclonal antibody (green).

Orexin B and p38 alpha were detected in frozen sections of mouse brainstem using mouse anti-human Orexin B monoclonal antibody (red) and rabbit anti-human/mouse/rat p38 alpha affinity-purified polyclonal antibody (green).