Product Details
Catalog Number: RA22104
Applications: ICC, WB, IF
Type: Rabbit IgG
Dilutions: ICC 1:5000; WB 1:10,000
Immunogen: Pig intermediate filaments were prepared from spinal cords by the method of Delacourte et al
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Whole serum
Format B: liquid
Species Reactivity: Human, Mouse, Rat, Chicken, Cat
Entrez: 4747
UniProt: P07196
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
100 ul$275.00Add to Cart

Neurofilaments can be defined as the intermediate or 10nm filaments found in specifically in neuronal cells. In the electron microscope neurofilaments appears as 10nm diameter fibres of indeterminate length which generally have fine wispy protrusions from their sides. They are found particularly abundantly in axons of large projection neurons. Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons, and are biochemically very stable.

NF-L antibody can also be useful in the diagnostics of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease and Alzheimer's disease. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease.

Images

Mixed neuron/glia cultures from newborn rat brain stained with antibody to peripherin (green) and rabbit polyclonal antibody NF-L (red channel). The blue channel reveals the localization of DNA.

Mixed neuron/glia cultures from newborn rat brain stained with antibody to peripherin (green) and rabbit polyclonal antibody NF-L (red channel). The blue channel reveals the localization of DNA.

Western blot of whole rat cerebellum homogenate stained NF-L in the left strip, at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~68kDa corresponds to rodent NF-L.

Western blot of whole rat cerebellum homogenate stained NF-L in the left strip, at dilution of 1:20,000. A prominent band running with an apparent SDS-PAGE molecular weight of ~68kDa corresponds to rodent NF-L.