Product Details
Catalog Number: RA22107
Applications: WB, IHC, IF
Type: Rabbit IgG
Immunogen: peptide corresponding to the C terminus of MMP2, specifically MGPLLVATFWPELPEK, which was coupled to keyhole limpet hemocyanin with glutaraldehyde.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Rat
Entrez: 4313
UniProt: P08253
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$295.00Add to Cart

The Immunogen: Matrix metalloproteinase-2 (MMP2) (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase), a member of the matrix metalloproteinase (MMP) enzyme family, is involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. MMP2 degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response. Mutations in this gene have been associated with Winchester syndrome, Torg syndrome and nodulosis-arthropathy-osteolysis syndrome.

MMP2 is secreted as an inactive proenzyme/zymogen which is activated when cleaved by extracellular proteinases. The proform has a molecular weight of 72kDa and the active form 62kDa. A C-terminus peptide (aa. 475-490) was selected which is highly conserved and unique to MMP2, and is present in both pro- and active forms. The peptide was conjugated to KLH and use to immunize rabbits. The hyperimmune serum was affinity purified on a peptide-coupled sepharose column. The purified immunoglobulin recognizes 72 kDa and 66 kDa bands corresponding to MMP2 on Western immunoblots. Some tissue extracts also show MMP2 complexes and degradation products. Polyclonal MMP2/475 antibody immunostains MMP2 specifically in various tissue preparations including paraffin sections.

Images

MMP-2 staining of cross-section of injured rat nerve showing MMP2 expression by Schwann cells and other reactive cells.

MMP-2 staining of cross-section of injured rat nerve showing MMP2 expression by Schwann cells and other reactive cells.

Blots of recombinant human MMP2 both pro and active forms (left lane) and crude homogenate from injured rat peripheral nerve (right lane) immunolabeled with MMP-2 antibody.

Blots of recombinant human MMP2 both pro and active forms (left lane) and crude homogenate from injured rat peripheral nerve (right lane) immunolabeled with MMP-2 antibody.