Product Details
Catalog Number: RA22124
Applications: ICC, WB, IHC, IF
Type: Rabbit IgG
Dilutions: Immunofluorescent: 1:5,000 Immunocytochemistry: 1:5,000 Immunohistochemistry: 1:5,000 Western Blot: 1:10,000
Immunogen: Full length recombinant human vimentin protein expressed in and purified from E. coli.
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: liquid
Species Reactivity: Human, Mouse, Rat, Rabbit, Bovine, Equine
Entrez: 7431
UniProt: P08670
Downloads: Datasheet (pdf)
Product Sizes
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100 ul$303.00Add to Cart

Vimentin is a protein which assembles to produce 10mm or intermediate filaments, which are major components of the cellular cytoskeleton. This protein is expressed in microglia, developing astrocytes, developing neurons, fibroblasts and endothelial cells in the developing nervous system. Levels of vimentin generally are reduced as development proceeds and in adult animals vimentin is mostly found in mesenchymal tissues. Antibodies to vimentin are useful in studies of stem cells and generally to reveal the filamentous cytoskeleton. The immunogen used to generate our antibody was recombinant human vimentin expressed in and purified from E. coli.

Images
Immunofluorescence

Immunofluorescence analysis of HeLa cells costained with rabbit pAb to vimentin, RA22124, dilution 1:5,000, in green, and mouse mAb to Actin dilution 1:500, in red. Blue is DAPI staining of nuclear DNA. The vimentin antibody stains the 10nm or intermediate filament network of the cytoskeleton. The antibody to actin labels the submembranous actin-rich cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites

Immunofluorescence

Immunofluorescence analysis of HeLa cells costained with rabbit pAb to vimentin, RA22124, dilution 1:5,000, in green, and mouse mAb to Actin dilution 1:500, in red. Blue is DAPI staining of nuclear DNA. The vimentin antibody stains the 10nm or intermediate filament network of the cytoskeleton. The antibody to actin labels the submembranous actin-rich cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites

Western Blot

Western blot analysis of whole cell lysates using rabbit pAb to vimentin, RA22124, dilution 1:5,000, in green. [1] protein standard (red), [2] HeLa, [3] SH-SY5Y, [4] HEK293, [5] NIH-3T3 cells. Strong band corresponds to vimentin protein with apparent SDS-PAGE molecular weight of ~55kDa

Western Blot

Western blot analysis of whole cell lysates using rabbit pAb to vimentin, RA22124, dilution 1:5,000, in green. [1] protein standard (red), [2] HeLa, [3] SH-SY5Y, [4] HEK293, [5] NIH-3T3 cells. Strong band corresponds to vimentin protein with apparent SDS-PAGE molecular weight of ~55kDa