Vector backbone contains two sequencing primer sites and two PvuII restriction enzyme cut sites.
The EF-1 Alpha promoter provides a stable, high-level of expression in most all mammalian cells. A great promoter to create brightly fluorescing cells and robust drug resistant cell lines.
The multiple cloning site (MCS) allows for the insertion of desired genes into our Sleeping Beauty transposon vectors. We follow our MCS with an IRES element to ensure consistent expression of both MCS inserted cargo and reporter genes. MCS site is prepped for digest with EcoRI and EcoRV restriction enzymes.
Green Fluorescent Protein reporter expression enables the identification of cells expressing your desired MCS cargo