Vector backbone contains two sequencing primer sites and two PvuII restriction enzyme cut sites.
The elongation factor 1α short, EFS, promoter is the miniaturized version of EF1α promoter. This makes it an ideal promoter when keeping your plasmids small is necessary for viable transfection into difficult cells.
Enhanced Green Fluorescence Protein is a bright, rapidly active reporter gene. It is useful as both a marker for flow cytometry and fluorescence microscopy.