Vector backbone contains two sequencing primer sites and two PvuII restriction enzyme cut sites.
The elongation factor 1α short, EFS, promoter is the miniaturized version of EF1α promoter. This makes it an ideal promoter when keeping your plasmids small is necessary for viable transfection into difficult cells.
The multiple cloning site (MCS) allows for the insertion of desired genes into our Sleeping Beauty transposon vectors. We follow our MCS with an IRES element to ensure consistent expression of both MCS inserted cargo and reporter genes. MCS site is prepped for digest with EcoRI and EcoRV restriction enzymes.
Puromycin resistance gene enables drug selection of transposon containing cells.