Vector backbone contains two sequencing primer sites and two PvuII restriction enzyme cut sites.
The elongation factor 1α short, EFS, promoter is the miniaturized version of EF1α promoter. This makes it an ideal promoter when keeping your plasmids small is necessary for viable transfection into difficult cells.
Red Fluorescent Protein emits an orange/red light when excited with 553-575nm light. It is ideal as a rapid acting fluorescence reporter.