Vector backbone contains two sequencing primer sites and two PvuII restriction enzyme cut sites.
The Ubiquitin C promoter is a staple choice for generating transgenic cell lines with stable consistent expression across a wide variety of mammalian species.
The multiple cloning site (MCS) allows for the insertion of desired genes into our Sleeping Beauty transposon vectors. We follow our MCS with an IRES element to ensure consistent expression of both MCS inserted cargo and reporter genes. MCS site is prepped for digest with EcoRI and EcoRV restriction enzymes.
Green Fluorescent Protein reporter expression enables the identification of cells expressing your desired MCS cargo.