Human Mesenchymal Stem Cells are isolated from umbilical cord blood are permanently stained with Hoechst, which intercalates into the DNA and cryopreserved. These cells are dervived from native human MSCs. They are suitable for a variety of basic research studies of self-renewal and differentiation. They are expandable through 5-10 passages. They expands in Low Serum, Complete Medium (Cat. No. SC00B1); which is optimized for high growth rates, reduced doubling times, healthy cells and stability
Optimizing your hMSC Cultures:
1) Grow cells in 1 to 5% O2, 5% CO2, with the balance of the gas content as N2..
2) Use of our MSCGroTM media is highly recommended.
3) Substrate- We recommend Greiner Bio-one TC-treated cell culture flasks or equivalent.
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