Product Details
Catalog Number: SC00A5
Applications: Cell Assays
Type: Primary Cells
Storage: Keep frozen in liquid nitrogen until plating
Format A: Frozen
Species Reactivity: Human
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
500,000 Cells$655.00Add to Cart

These human fibroblast cells are suitable for use in derivation of iPS cells and other applications requiring human fibroblasts including vaccine production and studies of cancer-associated fibroblasts (CAFs). They are expandable through 18 passages (~ 50 population doublings) and maintain strong growth while growth rates slowly decrease at later passages. They are provided as >500,000 cryopreserved cells.

500,000 cells

Authenticated human by karyotyping and human actin, Cytochrome B and COX1 PCR. Human by DNA profiling.

Indefinitely stable in liquid nitrogen.

Virus (HBV, HCV, HIV-1 & 2) and mycoplasma negative by PCR. >80% viability by PI. Passes growth specifications.

Product is manufactured, tested and validated in an ISO 9001/ISO13485/CLIA certified facility. 

This product requires shipment on dry ice and immediate transfer to liquid nitrogen storage or culture.

Images

Pancreatic Fibroblasts in culture

Pancreatic Fibroblasts in culture

Pancreatic Fibroblasts (SC00A5- stained with rhodamine phalloidin(Rh-Ph)+dapi)

Pancreatic Fibroblasts (SC00A5- stained with rhodamine phalloidin(Rh-Ph)+dapi)

Pancreatic Fibroblasts (SC00A5- stained with rhodamine phalloidin(Rh-Ph)+dapi)

Pancreatic Fibroblasts (SC00A5- stained with rhodamine phalloidin(Rh-Ph)+dapi)

Pancreatic stellate cells are positive for GFAP and a -SMA. Immunofluorescence analysis of representative primary PSC lines grown on chamber slides. Cells were stained with 40, 6-diamidino-2-phenylindole (DAPI; blue) and antibodies (green) for vimentin, GFAP, and alpha-SMA. The human PANC-1 cell and HPF line were negative controls for alpha-SMA staining. Slides were visualized at 40 magnification. Published OnlineFirst March 20, 2013; doi: 10.1158/0008-5472.CAN-12-4601. Cancer Res May 15, 2013 73; 3007.

Pancreatic stellate cells are positive for GFAP and a -SMA. Immunofluorescence analysis of representative primary PSC lines grown on chamber slides. Cells were stained with 40, 6-diamidino-2-phenylindole (DAPI; blue) and antibodies (green) for vimentin, GFAP, and alpha-SMA. The human PANC-1 cell and HPF line were negative controls for alpha-SMA staining. Slides were visualized at 40 magnification. Published OnlineFirst March 20, 2013; doi: 10.1158/0008-5472.CAN-12-4601. Cancer Res May 15, 2013 73; 3007.