Product Details
Product No Longer Available - Contact david@neuromics.com to learn more These human fibroblast cells are suitable for use in derivation of iPS cells and other applications requiring human fibroblasts including vaccine production and studies of cancer-associated fibroblasts (CAFs). They are expandable through 18 passages (~ 50 population doublings) and maintain strong growth while growth rates slowly decrease at later passages. They are provided as >500,000 cryopreserved cells.
Product is manufactured, tested and validated in an ISO 9001/ISO13485/CLIA certified facility. This product requires shipment on dry ice and immediate transfer to liquid nitrogen storage or culture. |
- Product ReviewsProduct is great quality Product Name: Native Human Fibroblasts - Pancreas Derived (Cat# SC00A05) http://bit.ly/1e7ilVO Organization: Fox Chase Cancer CenterTiffany luong - Oct 30, 2013 - Rating: 5.0
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- Product Publications
Product Publications
- Andreas Stylianou, Vasiliki Gkretsi, Maria Louca, Lefteris C. Zacharia, and Triantafyllos Stylianopoulos (2019). Collagen content and extracellular matrix cause cytoskeletal remodelling in pancreatic fibroblasts. J. R. Soc. Interface 16: 20190226. http://dx.doi.org/10.1098/rsif.2019.0226
- Maria Kalli, Panagiotis Papageorgis, Vasiliki Gkretsi, Triantafyllos Stylianopoulos. (2018). Solid Stress Facilitates Fibroblasts Activation to Promote Pancreatic Cancer Cell Migration. Annals of Biomedical Engineering. https://doi.org/10.1007/s10439-018-1997-7
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Images
Pancreatic Fibroblasts in culture
Pancreatic Fibroblasts in culture
Pancreatic Fibroblasts (SC00A5- stained with rhodamine phalloidin(Rh-Ph)+dapi)
Pancreatic Fibroblasts (SC00A5- stained with rhodamine phalloidin(Rh-Ph)+dapi)
Pancreatic stellate cells are positive for GFAP and a -SMA. Immunofluorescence analysis of representative primary PSC lines grown on chamber slides. Cells were stained with 40, 6-diamidino-2-phenylindole (DAPI; blue) and antibodies (green) for vimentin, GFAP, and alpha-SMA. The human PANC-1 cell and HPF line were negative controls for alpha-SMA staining. Slides were visualized at 40 magnification. Published OnlineFirst March 20, 2013; doi: 10.1158/0008-5472.CAN-12-4601. Cancer Res May 15, 2013 73; 3007.
Pancreatic stellate cells are positive for GFAP and a -SMA. Immunofluorescence analysis of representative primary PSC lines grown on chamber slides. Cells were stained with 40, 6-diamidino-2-phenylindole (DAPI; blue) and antibodies (green) for vimentin, GFAP, and alpha-SMA. The human PANC-1 cell and HPF line were negative controls for alpha-SMA staining. Slides were visualized at 40 magnification. Published OnlineFirst March 20, 2013; doi: 10.1158/0008-5472.CAN-12-4601. Cancer Res May 15, 2013 73; 3007.