Image: Human adipose derived mesenchymal stem cells were expanded for 10 passages. Cells were plated at 5,000/cm² in a Greiner Bio-One T75 flask and maintained in MSC-GRO low serum, complete medium (Vitro Biopharma Cat. No. SC00B1) in a reduced O₂ environment (1%O₂, 5%CO₂, 90%N₂) at 37°C in a humidified chamber. The AD-MSCs. harvested and counted on a Beckman-Coulter Z2 particle counter (range 10-30uM). Doubling time of 23.73hrs was calculated using ln(2*dT)/ln(Cf/Ci), where dT is the time, in hours, from inoculation to detachment; Ci is the initial number of cells plated and Cf is the final number of cells recovered from subculture.
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Name Catalog # Size Hoechst-labeled Human Mesenchymal Stem Cells SC00A1-HC 500,000 Cells Human Mesenchymal Stem Cells SC00A1 500,000 Cells Human Mesenchymal Stem Cells - Bone Marrow Derived MSC001 500,000 Cells Human Mesenchymal Stem Cells Expressing Oct3/4 SC00A6 500,000 Cells Human Mesenchymal Stem Cells-Green Fluorescent Nanoparticle-labeled SC00A4NP 500,000 Cells Human Mesenchymal Stem Cells-Green Fluorescent-Labeled SC00A2 500,000 Cells Human Mesenchymal Stem Cells-Red Fluorescent-Labeled SC00A3 500,000 Cells