Collagen is a fibrous protein found in the extracellular matrix and connective tissue. The most common form of collagen is type I and is most prevalent in bone, tendon and skin. It consists of 3 intertwined coiled subunits: 2 x α1 (I) chains and 1 x α2 (I) chain. Each chain contains 1050 amino acids wound tightly around one another in a characteristic right-handed triple helix. The triple-helical structure of collagen arises from unique abundance of the amino acids in collagen appear in a characteristic repeating motif Gly-X-Y, where X is usually proline and Y is usually hydrozyproline.
Smooth Coat Solution (SC300) is a biocompatible complex of extracellular matrix binding solution that helps accelerate cell attachment in ≤ 7 minutes. SC300 is a purified GMP grad solution in which cells proliferate and stabilize.
SC300 is ideal for plate coating due to its unique viscosity. Its coating greatly enables cell migration on cultured plate surfaces. Perfect for establishing primary cell lines, it can increase endothelial cell attachment and survival in culture.
For culturing cells from frozen, we recommend using AlphaBioCoat, our premium smooth coat solution, that contains growth factors to help facilitate cell growth.
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