These reagents are selected to address the expressed needs of our customer research in areas such as Degenerative Diseases, Oxidative Stress and Oncology.

Our apoptosis detection kits are designed to work in vivo and in vitro.

Jurkat cells dually stained with Hoechst and Polycaspase Assay Kit, green--FAM-VAD-FMK. Caspase activity is revealed by green fluorescence in cell #2, indicating that only this cell is apoptotic. Cell #1 is also dying (scattered blue), but is not apoptotic because it is not green. Cell #3 is healthy (concentrated blue nucleus).
  • FLICA® In Vitro Caspase Kits

    Easy to use, caspase inhibitor-based apoptosis detection kits that have been tested in primary neurons and many other cell types! Features:

    •  Easy-Just add the reagent directly to the cell culture and incubate (see sidebar).
    •  Fast-The reactions start within 15 minutes of addition to the cells, however we recommend an incubation of 1-4 hours. 
    •  Accurate-There is no interference from pro-caspases or inactive forms of the enzyme. Quantitative Read with a fluorescence plate reader, microscope, or flow cytometer.
    •  Reliable-Only cells with active caspase enzymes fluoresce. The reagents may be incubated up to 72 hours without killing the cells
    •  Sensitive-Detects background levels of apoptosis in controls. Detect apoptosis earlier than Annexin V and TUNEL Caspases are released before the phospholipid turnover & DNA laddering.

    Name Catalog # Type Species Applications Size Price
    660 Caspase-1 Assay Kit KF17362 Kit All Apoptosis Detection 25-50 Tests $229.00
    660 Polycaspase Assay Kit KF17361 Kit All Apoptosis Detection 25-50 Tests $229.00
    Caspase 1 Assay Kit, green KF17201 Kit All Apoptosis Detection 100 Tests $592.00
    Caspase 1 Assay Kit, green KF17201 Kit All Apoptosis Detection 25 Tests $211.00
    Caspase 10 Assay Kit, green KF17207 Kit All Apoptosis Detection 100 Tests $561.00
    Caspase 10 Assay Kit, green KF17207 Kit All Apoptosis Detection 25 Tests $195.00
    Caspase 13 Assay Kit, green KF17208 Kit All Apoptosis Detection 100 Tests $561.00
    Caspase 13 Assay Kit, green KF17208 Kit All Apoptosis Detection 25 Tests $195.00
    Caspase 2 Assay Kit, green KF17202 Kit All Apoptosis Detection 100 Tests $561.00
    Caspase 2 Assay Kit, green KF17202 Kit All Apoptosis Detection 25 Tests $195.00
    Caspase 3 & 7 Assay Kit, green KF17203 Kit All Apoptosis Detection 100 Tests $561.00
    Caspase 3 & 7 Assay Kit, green KF17203 Kit All Apoptosis Detection 25 Tests $195.00
    Caspase 6 Assay Kit, green KF17204 Kit All Apoptosis Detection 100 Tests $561.00
    Caspase 6 Assay Kit, green KF17204 Kit All Apoptosis Detection 25 Tests $195.00
    Caspase 8 Assay Kit, green KF17205 Kit All Apoptosis Detection 100 Tests $561.00
    Caspase 8 Assay Kit, green KF17205 Kit All Apoptosis Detection 25 Tests $195.00
    Caspase 9 Assay Kit, green KF17206 Kit All Apoptosis Detection 100 Tests $561.00
    Caspase 9 Assay Kit, green KF17206 Kit All Apoptosis Detection 25 Tests $195.00
    Caspase 9 Assay Kit, red KF17302 Kit All Apoptosis Detection 100 Tests $561.00
    Caspase 9 Assay Kit, red KF17302 Kit All Apoptosis Detection 25 Tests $195.00
    Polycaspase Assay Kit, green KF17200 Kit All Apoptosis Detection 100 Tests $561.00
    Polycaspase Assay Kit, green KF17200 Kit All Apoptosis Detection 25 Tests $195.00
    Polycaspase Assay Kit, red KF17300 Kit All Apoptosis Detection 100 Tests $195.00
    Polycaspase Assay Kit, red KF17300 Kit All Apoptosis Detection 25 Tests $179.00
  • Necrosis vs. Apoptosis Assay Kits

    Our Necrosis vs Apoptosis Assay kit simultaneously detects both apoptosis associated cytotoxicity events as well as cell death due to necrosis. Apoptotic cells are identified using ICT’s Fluorescent Labeled Inhibitor of CAspases (FLICA) reagent probe. The FAM-FLICA probe covalently binds to active caspase enzymes, which are up-regulated during apoptosis, thus clearly labeling apoptotic cells for subsequent analysis. Non-apoptotic cells will not contain the active caspase enzymes required for FAM-FLICA to remain covalently bound within the cell structure. 

    Name Catalog # Type Species Applications Size Price
    Necrosis vs Apoptosis Kits KF17371 Apoptosis Detection 50-100 Tests $295.00
    Necrosis vs Apoptosis Kits KF17372 Apoptosis Detection 100-200 Tests $550.00
  • FLISP™ Serine Protease Detection Kits

    The chymotrypsin-like serine rotease subfamily plays a key role in a number of diverse physiological processes, including the maintenance of homeostasis, inflammation and immune reactions, apoptosis, and cell survival. For example,chymotrypsin-like serine proteases have been shown to be activated during apoptosis signaling. Additionally, some chymotrypsin serine protease inhibitors have been shown to induce apoptosis by blocking IkBa degradation, which is important in the NFkB cell survival signaling pathway.

    Use our FFCK kit to detect chymotrypsin-like serine proteases that favor phenylalanine;use the FLCK kit for those that favor leucine; use the kits with an additional spacer sequence to reduce steric hindrance at the binding site of the serine protease.

    Name Catalog # Type Species Applications Size Price
    FAM-Leu-CMK FLISP™ Assay Kit KF17317 Kit 100 Tests $494.00
    FAM-Lue-CMK FLISP™ Assay Kit KF17316 Kit 25 Tests $185.00
    FAM-Phe-CMK FLISP™ Assay Kit KF17314 Kit 25 Tests $185.00
    FAM-Phe-CMK FLISP™ Assay Kit KF17315 Kit 100 Tests $494.00
    FAM-Spacer-Leu-CMK FLISP™ Assay Kit KF17324 Kit 25 Tests $185.00
    FAM-Spacer-Phe-CMK FLISP™ Assay Kit KF17322 Kit 25 Tests $185.00
    FAM-Spacer-Phe-CMK FLISP™ Assay Kit KF17323 Kit 100 Tests $494.00
    SR-101-Leu-CMK FLISP™ Assay Kit KF17321 Kit 100 Tests $494.00
    SR-101-Phe-CMK FLISP™ Assay Kit KF17318 Kit 25 Tests $185.00
    SR-101-Phe-CMK FLISP™ Assay Kit KF17319 Kit 100 Tests $494.00
  • Magic Red™ Real Time! Kits
    Magic Red™  (MR) kits measure apoptosis via active caspases and cathepsins in whole living, intact cells - no lysis required. As apoptosis progresses and caspase activity increases, the red fluorescent signal increases. These unique kits are not ELISAs and do not use antibodies - instead they are based on cell-permeant substrate reagents specifically targeted by active apoptosis enzymes. 
        

    Just culture your cells, add MR to the media, and incubate. If the enzymes are active, they will cleave the reagent causing it to fluoresce red. MR kits are true substrate assays - the red fluorescent label is quenched until it is actually cleaved by active enzymes. Only cells with active enzymes will fluoresce, so you don’t get any signal from pro-enzymes or inactive forms of the enzyme.

    Name Catalog # Type Species Applications Size Price
    Magic Red™ Caspases 3&7 KF17306 Kit 25 Tests $180.00
    Magic Red™ Caspases 3&7 KF17307 Kit 100 Tests $494.00
    Magic Red™ Cathepsin B KF17308 Kit 25 Tests $185.00
    Magic Red™ Cathepsin B KF17309 Kit 100 Tests $494.00
    Magic Red™ Cathepsin K KF17310 Kit 25 Tests $185.00
    Magic Red™ Cathepsin L KF17312 Kit 25 Tests $185.00
    Magic Red™ Cathepsin L KF17313 Kit 100 Tests $494.00
  • MitoPT™ Kits

    These Kits easily assess changes in mitochondrial membrane potential. Changes in mitochondrial membrane potential can correlate with cytochrome c release and the initiation of apoptosis.

    Name Catalog # Type Species Applications Size Price
    MitoPT™ JC-1 green and red fluorescence KF17305-100 Kit 100 Tests $205.00
    MitoPT™ JC-1 green and red fluorescence KF17305-400 Kit 400 Tests $449.00
    MitoPT™ TMRE & TMRM Kits KF17358-500---TMRE Kit 500 Test $329.00
  • Cytotoxicity Test Kits

    Cytolytic activity is an important process by which the immune system eliminates compromised cells in the body. Whether describing antibody-dependent cell-mediated cytotoxicity (ADCC), complement-mediated cytotoxicity, or lymphocyte-mediated cytotoxicity, the activation of the immune system leads to the removal of target cells infected by pathogens or transformed by cancer. Alternatively, cytotoxicity can simply refer to any compound (drug or toxin) that also results in killing of the target cells.

    We offer two easy, reliable, and accurate assays to measure cytotoxicity. The Basic Cytotoxicity Test simplifies measurement of necrotic and membrane-compromised cells. The Total Cytotoxicity Test  further distinguishes apoptosis from necrosis, leading to a more accurate assessment of cytolytic activity. At least 10% more cytolytic activity is often measured when apoptotic cells are also identified. ICT's kits provide a direct measurement of cytotoxicity rather than using an indirect indicator, such as the release of LDH or ATP enzymes, and there is no need to lyse the cells. These assays do not use any radioisotopes, they are much safer and more cost effective to run than 51Cr assays. Highlights:

    • Easy - run samples in a single tube
    • Safe – no radioactivity
    • Fast - finish in one day Sensitive - measure killed and apoptotic cells
    • Accurate – analyze cells with a flow cytometer
    Name Catalog # Type Species Applications Size Price
    Basic Cytotoxicity Test Kits CT17001 Kit Not Applicable FC 125 Tests $275.00
    Basic Cytotoxicity Test Kits CT17002 Kit 250 Tests $460.00
    Total Cytotoxicity Test Kits CT17003 Kit Not Applicable FC 125 Tests $355.00
    Total Cytotoxicity Test Kits CT17004 Kit 250 Tests $585.00
  • Autophagy Assay Kits

    Autophagy is a conserved lysosomal recycling process by which cells break down their own components such as proteins, lipids, and carbohydrates. The process plays an important role in maintaining homeostasis and destroying intracellular pathogens. In addition, autophagy can be upregulated in times of starvation or stress to provide additional nutrients for the cell. Dysregulation of autophagy has implications in cancer, infection, and degenerative diseases.

    Autophagy is a three stage process. First, cytoplasmic components targeted for degradation are sequestered, resulting in the formation of the autophagosome. Next, the autophagosome fuses with the lysosome to form the autophagolysosome or autolysosome. Finally, degradation of the autophagosomal contents occurs.

    Our Autophagy Assay, Red (cat# KF17373) enables researchers to detect and monitor the in vitro development of autophagy in living cells. The Autophagy Probe is cell-permeant and fluoresces red when inserted in the lipid membranes of autophagosomes and autolysosomes. Results can be read using a flow cytometer.

    Name Catalog # Type Species Applications Size Price
    Autophagy Assay, Red KF17373-200 200 tests $375.00
    Autophagy Assay, Red KF17373-50 Kit Autophagy Detection 50 Tests $175.00