Product Details
Catalog Number: ELH-AgRP-003
Applications: E
Type: Kit
Species Reactivity: Human
Downloads: Datasheet (pdf)
Product Sizes
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5 Plate Kit$1,499.00Add to Cart

Sensitive, Specific and Cost Effective

Agouti-related Protein (AgRP), also known as the protein product of the agouti-related transcript (ART), is a neuropeptide and a functional antagonist of alpha-MSH on MC-3 and MC-4 receptors, both of which are implicated in obesity. AgRP shares sequence and structural homology with the Agouti protein and has similar expression patterns in both mouse and human

The RayBio® Human AgRP ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human AgRP in serum (human AgRP concentration is pretty low in normal serum/plasma, it may not be detected in this assay), plasma, cell culture supernatants and urine. /span>

Image: AgRP Standard Curve

Suggested Application:Quantitative Protein Detection; Establishing Normal Range; Validation of Antibody Array Results.

Kit Components:
·   Pre-Coated 96-Well Strip Microplate
·   Wash Buffer
·   Stop Solution
·   Assay Diluent(s)
·   Lyophilized Standard
·   Biotinylated Detection Antibody
·   Streptavidin-Conjugated HRP
·   TMB One-Step Substrate
Other Materials Required:
·     Distilled or deionized water
·     Precision pipettes to deliver 2 μl to 1 ml volumes
·     Adjustable 1-25 ml pipettes for reagent preparation
·     100 ml and 1 liter graduated cylinders
·     Tubes to prepare standard and sample dilutions
·     Absorbent paper
·     Microplate reader capable of measuring absorbance at 450nm
·     Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline:
1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C.
3.Add 100 μl of prepared biotin antibody to each well. Incubate 1 h at RT.
4.Add 100 μl of prepared Streptavidin solution to each well. Incubate 45 min at RT.
5.Add 100 μl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT.
6.Add 50 μl of Stop Solution to each well. Read at 450 nm immediately.
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