Product Details
Catalog Number: ELH-GDNF-001
Applications: E
Type: Kit
Species Reactivity: Human, Primate
Downloads: Datasheet (pdf)
Product Sizes
SizeList PricePriceCart
1 Plate Kit$559.00Add to Cart
5 Plate Kit$1,999.00Add to Cart

Sensitive, Specific and Cost Effective

Glial cell-derived neurotrophic factor (GDNF) is a small protein that potently promotes the survival of many types of neurons. GDNF has regenerative properties for brain cells and showed potential as treatment for Parkinson's and related neurodegenerative diseases

The RayBio® Human BDNF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human, primate and rat BDNF in serum, plasma, cell culture supernatants and urine.

Image: GDNF Standard Curve

Suggested Application:Quantitative Protein Detection; Establishing Normal Range; Validation of Antibody Array Results.

Kit Components:
·   Pre-Coated 96-Well Strip Microplate
·   Wash Buffer
·   Stop Solution
·   Assay Diluent(s)
·   Lyophilized Standard
·   Biotinylated Detection Antibody
·   Streptavidin-Conjugated HRP
·   TMB One-Step Substrate
Other Materials Required:
·     Distilled or deionized water
·     Precision pipettes to deliver 2 μl to 1 ml volumes
·     Adjustable 1-25 ml pipettes for reagent preparation
·     100 ml and 1 liter graduated cylinders
·     Tubes to prepare standard and sample dilutions
·     Absorbent paper
·     Microplate reader capable of measuring absorbance at 450nm
·     Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline:
1.Prepare all reagents, samples and standards as instructed in the manual.
2.Add 100 μl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C.
3.Add 100 μl of prepared biotin antibody to each well. Incubate 1 h at RT.
4.Add 100 μl of prepared Streptavidin solution to each well. Incubate 45 min at RT.
5.Add 100 μl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT.
6.Add 50 μl of Stop Solution to each well. Read at 450 nm immediately.
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