These kits are designed for in vitro dopaminergic neuron differentiation of ES cells in a serum-free environment. The kits contain ITS and N-2 Supplements, which are used to select and enrich neural stem cell populations. Bovine Fibronectin is included to support for cell attachment and spreading. A growth factor panel, consisting of human fibroblast growth factor basic (FGF basic), mouse fibroblast growth factor 8b (FGF-8b) and mouse sonic hedgehog amino-terminal peptide (SHH-N), is included for effective dopaminergic differentiation. The quantity of each component provided in the kit is estimated to be sufficient for induction of 3 x 107 ES cells.