Product Details
Catalog Number: GT15219
Applications: WB, IHC, E
Type: Goat IgG
Immunogen: Purified, E. coli-derived, recombinant human Mothers Against DPP homolog 1 (rhSmad1; aa 2 - 454).
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Rat
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$365.00Add to Cart

SMAD1 is a member of the Mothers Against Dpp (MAD)-related family of proteins. So far, eight SMADs have been identified and can be divided into 3 subgroups based on their structure and function; pathway-restricted, common mediator and inhibitory. SMAD1, 5 and 8 serve as pathway-restricted SMADs for the bone morphogenetic protein (BMP) signaling pathway.

Smad1 is phosphorylated resulting in its association with the common-mediator subunit, Smad4. This heteromeric complex then translocates into the nucleus to exert transcriptional comodulator activity.

Images

Detection of Smad1. Lysates from 5 x 10^5 human HepG2, MDA-MB-468, and HT29 cells were resolved by SDS-PAGE, transferred to Immobilon-P membrane, and immunoblotted with 0.5 μg/mL goat anti-Smad1 as described in Protocols for Immunoblotting. A 30 second exposure to film is shown.

Detection of Smad1. Lysates from 5 x 10^5 human HepG2, MDA-MB-468, and HT29 cells were resolved by SDS-PAGE, transferred to Immobilon-P membrane, and immunoblotted with 0.5 μg/mL goat anti-Smad1 as described in Protocols for Immunoblotting. A 30 second exposure to film is shown.