FAS Ligand, also known as CD95L or APT1LG1 , is a member of the TNF receptor family.
The full length Fas-L protein has a cytoplasmic domain, transmembrane (Type II) domain and extracellular domain. The membrane-bound form of Fas-L is proteolytically cleaved to produce soluble Fas-L. There are at least 2 known isoforms of Fas-L produced by alternate splicing.
Fas ligand-receptor interactions play an important role in the regulation of the immune system and the progression of cancer.
Figure 1-The biological activity of rhFas Ligand was measured by its ability to induce apoptosis of Jurkat cells (Cifone M.G. et al., 1994, J. Exp. Med. 180:1547). The ED50 of this effect is typically 0.2 - 0.5 ng/mL.
Figure 2-To measure the ability of the antibody to neutralize the bioactivity of rhFas Ligand, rhFas Ligand was incubated with various concentrations of the antibody for 1 hour at 37° C. Following this preincubation period, Jurkat cells were added. The assay mixture in a total volume of 100 μL/well, containing antibody at the concentrations indicated, rhFas Ligand at 2 ng/mL and Jurkat cells at 5 x 104/mL, was incubated for 2 days at 37° C in a 5% CO2 humidified incubator. 3H-thymidine was added during the final 4 hours of the incubation. The cells were subsequently harvested onto a glass fiber filter and the 3H-thymidine incorporation into DNA was determined. The ND50 of this antibody is typically 2 - 8 ng/mL.
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Name Catalog # CD11b (OX-42) CH23021 Prion Protein/CD230 CH23027 ACE/CD143 FC15000 CD45 FC15008 IGF-I R/CD221-Fluorescein Labeled FC15010 gp130/CD130-Phycoerythrin Labeled FC15014 Endoglin/CD105 Fluorescein FC15022 CD39 GP10103 TRAIL R1/CD261 GT15061 TRAIL R3/CD263 GT15062 TRAIL R4/CD264 GT15063 PDGF R Beta/CD140B GT15065 gp130/CD 130 GT15072 ACE/CD143 GT15145 TIE2 GT15148 PDGF R Alpha/CD140A GT15150 IGF-II/C11orf43 GT15182 CD31/PECAM-1 GT15190 Frizzled 4/CD344 GT15217 CDCP1/CD318 GT15233 TLR2/CD282 GT15234 TLR4/CD284 GT15235 Endoglin/CD105 GT15247 FAS (CD95) MO15010 Neprilysin/CD10 MO15015 gp130/CD130 MO15024 IL-2 R alpha/CD35 MO15070 IGF-I R/CD221 MO15074 CCR1/CD191 MO15088 TLR3/CD283 MO15121 CEACAM-1/CD66a MO15123 Endoglin/CD105 MO15124 CD34/HPCA1 MO18004 CD45 MO18005 CD1a (Clone MT1B) MO20005 CD43 (Clone MT1) MO20006 CD44 Variant 3 MO20007 CD4 MO20025 CD34/HPCA1 MO20026 CD9/Motility-Related Protein-1 MO20046 Galectin-3 (LGAS3) MO22119 c-Kit/CD117 RA14132 CD36 RA25035 TLR9/CD289 RA25070 Macrophage Scavenger Receptor I/CD204 RA25074 FAS Ligand (CD95L) RA30043 TLR6/CD286 RA30053