Product Details
Catalog Number: GT15232
Applications: ICC, WB
Type: Goat IgG
Immunogen: E. coli derived recombinant mouse SLAIN1 Met262-Gln483 Accession # Q68FF7.
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Human, Mouse
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$365.00Add to Cart

SLAIN1 (SLAIN motifcontaining protein 1) is a 61 kDa (predicted) member of the SLAIN motif containing family of proteins. It is expressed in embryonic stem cells plus cells of the neural tube, and also appears in multiple adult tissues. Its function is unclear. Mouse SLAIN1 is 579 amino acids (aa) in length. It contains a coiledcoil region (aa 2156), a Prorich segment (aa 64160), and one Cterminal SLAIN (SerLeuAlaIleAsn) motif. There are two potential splice variants. One shows an alternate start site at Met262, while another shows a 50 aa insertion after Gln231. Over aa 262483, mouse SLAIN1 shares 96% and 92% aa identity with rat and human SLAIN1, respectively. 

Images

SLAIN1 staining of immersion fixed D3 mouse embryonic stem cell line (dilution of 10 μg/mL for 3 hours at room temperature). Cells were stained with the 557 fluoro-labeled conjugated AntiGoat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Protocol on Datasheet..

SLAIN1 staining of immersion fixed D3 mouse embryonic stem cell line (dilution of 10 μg/mL for 3 hours at room temperature). Cells were stained with the 557 fluoro-labeled conjugated AntiGoat IgG Secondary Antibody (red) and counterstained with DAPI (blue). Protocol on Datasheet..

Detection of Human/Mouse SLAIN1 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, human cerebellum tissue, and BG01V human embryonic stem cells. PVDF membrane was probed with 1 μg/mL of Human/Mouse SLAIN1 Antibody followed by HRPconjugated AntiGoat IgG Secondary Antibody. A specific band was detected for at approximately 60 kDa (as indicated). Protocol on Datasheet.

Detection of Human/Mouse SLAIN1 by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, human cerebellum tissue, and BG01V human embryonic stem cells. PVDF membrane was probed with 1 μg/mL of Human/Mouse SLAIN1 Antibody followed by HRPconjugated AntiGoat IgG Secondary Antibody. A specific band was detected for at approximately 60 kDa (as indicated). Protocol on Datasheet.