Product Details
Catalog Number: GT15239
Applications: ICC, WB, E, FC
Type: Sheep IgG
Immunogen: Purified, NS0-derived, recombinant mouse 5T4 extracellular domain (rm5T4; aa 30 - 361; Accession # AAH58198).
Storage: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Shipping: Frozen (Polar Packs)
Format A: Affinity Purified
Format B: liquid
Species Reactivity: Mouse
Entrez: 7162
UniProt: Q13641
Downloads: Datasheet (pdf)
Product Sizes
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100 ug$365.00Add to Cart

5T4 (oncofetal antigen 5T4; also TPBG and trophoblast glycoprotein) is a 72 - 80 kDa glycoprotein member of the LRR family of proteins. It is expressed on trophoblasts, tumor cells, ovarian cuboidal epithelium and differentiating embryonic stem cells and impacts cell adhesion and motility. The mouse 5T4 cDNA encodes a type I transmembrane protein precursor that is 426 amino acids (aa) in length. It contains a 332 aa extracellular region (aa 30 - 361) that shows one Ser-rich region followed by seven Leu-rich repeats (aa 90 - 338). Over aa 30 - 361, mouse 5T4shares 95% and 81% aa identity with rat and human 5T4, respectively.

Images
Detection of 5T4 by western blot.Mouse brain lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0 μg/mL of antibody.

Detection of 5T4 by western blot.Mouse brain lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0 μg/mL of antibody.

Detection of 5T4 by western blot.Mouse brain lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0 μg/mL of antibody.

Detection of 5T4 by western blot.Mouse brain lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1.0 μg/mL of antibody.

Differentiated D3 cells were stained with anti-5T4 (filled histogram)

Differentiated D3 cells were stained with anti-5T4 (filled histogram)

Differentiated D3 cells were stained with anti-5T4 (filled histogram)

Differentiated D3 cells were stained with anti-5T4 (filled histogram)