Product Details
Catalog Number: HN60000
Applications: Cell Assays
Type: Primary Cells
Storage: Keep frozen in liquid nitrogen until plating.
Shipping: Frozen (Dry Ice/Liquid Nitrogen)
Format A: Frozen
Species Reactivity: Human
Downloads: FAQs (pdf)
Downloads: Datasheet (pdf)
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1,000,000 Cells$795.00Add to Cart

hN2TM  Human Neurons are fully differentiated normal human neural cells derived as adherent cells from hESC WA09 line. The cells are shipped frozen in a vial with 1 x 106cells; once thawed they should be immediately plated on a Matrigel (or other suitable extracellular matrix protein)-coated dish and maintained in the accompanying serum-free medium.

Applications: Gene Expression Analysis, Western blotting, Flow Cytometry, Immunocytochemistry, FACS sorting, DNA Microarray, RT-PCR, Neurite Outgrowth assays, FLIPR calcium assays, cytotoxicity, and second messenger signaling. 

Research use only 

Kit Contents:

Component:

  • 1 vial cells
  • 1 L Phenol Free AB2TM Basal Medium
  • ANSTM Supplement

Storage Conditions

  • Liquid nitrogen
  • 2-8 ºC protected from light
  • <-20 ºC; if it thaws, store at 2-8 ºC; do NOT refreeze

Please note: There is enough media included to keep the cells viable for up to 1 month.

Required but Not Included:

  • 1 mL 100x Pen-Strep (Optional)
  • Coated plates or dishes with Matrigel® from BD
  • L-Glutamine (200 mM)-Invitrogen-25030081
  • Dulbecco's Modified Eagle's Medium
  • LIF-Millipore-LIF1010

DNA fingerprint cells:    The loci match the DNA fingerprint pattern for the H9 (NIH designation, WA09) HESC line as published in http://stemcells.nih.gov/research/nihresearch/scunit/.

Viral tests cells:  This lot was derived from the H9 hESC line that has been tested for Hepatitis B, Hepatitis C, HIV-1, HIV-2, HTLV-I/II, HSV1, HSV2, EBV, and CMV. The H9 cell line has been tested and shown to be negative. (Tests performed by GIVF Laboratories).

Delivery Time: 7-10 days

hN2 cells can produce inward currents that generate action potentials.

Figure: Isolated hN2 with significant neurite growth 1 week after plating . This cell was subjected to whole cell voltage clamp utilizing a potassium gluconate based intracellular solution. (B) Voltage gated inward and outward currents were elicited from this cell with depolarizing voltage steps. (C) Inward currents from another cell (potassium gluconate intracellular) were abolished by local application of 1 µM tetrodotoxin (red trace) while outward currents remained. Inward current recovered as TTX washed out of the region (green trace). (D) A different cell which exhibited voltage activated inward currents that inactivated in response to a 50 ms prepulse at different membrane potentials. The experiment was done 27 days after the removal of bFGF. A cesium gluconate based intracellular solution was used for this experiment to block outward potassium currents. The membrane potential for half maximal inactivation by standard Boltzman fitting (red line) was -40.1 mV with a slope of 4.7. (E) Recovery from fast inactivation utilizing a paired pulse protocol in the same cell as C. The single exponential time constant for recovery of inactivation was 1.7 ms (red line). (F) A different cell which elicited an overshooting action potential upon current injection under whole cell current clamp utilizing a potassium gluconate based intracellular solution. Inset: Response of the same cell under voltage clamp to a change in membrane potential from -80 mV to -10 mV elicited a peak current of 457 pA. Scale bars for inset: 5 ms, 0.2 nA.

 

Images

hN2 cells grown in culture for 4 days and stained with our chicken polyclonal to Neurofilament light or low molecular weight chain NF-L, a marker of neurons. Many of the differentiating cells show strong cytoplasmic and clearly fibrillar staining for NF-L. Blue stain is DAPI and reveals cell nuclei of some non neuronal cells in this culture.

hN2 cells grown in culture for 4 days and stained with our chicken polyclonal to Neurofilament light or low molecular weight chain NF-L, a marker of neurons. Many of the differentiating cells show strong cytoplasmic and clearly fibrillar staining for NF-L. Blue stain is DAPI and reveals cell nuclei of some non neuronal cells in this culture.

hN2 cells can produce inward currents that generate action potentials.

hN2 cells can produce inward currents that generate action potentials.

hN2 cells can produce inward currents that generate action potentials.

hN2 cells can produce inward currents that generate action potentials.