Product Details
Catalog Number: KF17309
Type: Kit
Downloads: Datasheet (pdf)
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100 Tests$494.00Add to Cart
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Lipopolysaccharide-primed J774A.1 cells were incubated with or without DCA (100 μM, 24 h); then, the cells were stained with Lyso Tracker Green DND-26 (Invitrogen) or Magic RedTM Cathepsin B fluorogenic substrate z-Arg-Arg cresyl violet (Neuromics) for 1 h, followed by Hoechst staining for half an hour. Fluorogenic signals were captured by inverted fluorescence microscope (Leica).

Lipopolysaccharide-primed J774A.1 cells were incubated with or without DCA (100 μM, 24 h); then, the cells were stained with Lyso Tracker Green DND-26 (Invitrogen) or Magic RedTM Cathepsin B fluorogenic substrate z-Arg-Arg cresyl violet (Neuromics) for 1 h, followed by Hoechst staining for half an hour. Fluorogenic signals were captured by inverted fluorescence microscope (Leica).

Detection of intracellular cathepsin K activity in THP-1 cells using (z-LR)2-MR-Cathepsin fluorogenic substrate

Detection of intracellular cathepsin K activity in THP-1 cells using (z-LR)2-MR-Cathepsin fluorogenic substrate