Nestin is a protein expressed in neural crest cells, CNS neural stems cells, as well as a few non-neural cell types in the embryo, including cells within the pancreatic islets of Langerhans and the limb bud. Nestin is an intermediate filament-associated protein, and contributes to the cytoskeleton.
Image: Immunostaining of hNP1 Progenitors before and after differentiation. The culture was highly homogenous with neural rosettes. (A) Neural rosettes (white arrows, bright field) from WA09 cells. A similar result also obtained with BG02 cell line. (B) Shown here, a neural rosette stained with Nestin (NES) (green) antibody. Propagated Neural progenitors showed expression of marker genes, NES (C) and Musashi 1 (D) but not SOX2 (E). Further differentiation produced neurons (Tuj1) (F), astrocytes (GFAP) (G) and oligodendrocytes (myelin basic protein)(H) (lower panel). DAPI (blue) was used for staining the nuclei (scale bar for (A) through (E) is 100 mm and for the remaining figures 10 mm). Differentiation (2007) DOI: 10.1111/j.1432-0436.2007.00256.x...Dilutions: NES (1:100, Neuromics, Edina, MN), MSI1 (1:100, Neuromics), Tuj1 (1:500, Neuromics)...
- Product ReviewsWe ordered some antibodies from Neuromics recently. In past we have ordered the same from different company, but the Neuromics antibodies works the best! We have been very satisfied with the results that we got using these antibodies and would recommend anyone working in this area to buy these from Neuromics. Product Name Nestin MAb (Clone 307501), Rat – (Cat# MO15056) http://bit.ly/WRr1bM Neurofilament NF-L, Rabbit – (Cat# RA22104) http://bit.ly/1rF1nCl ALDH1L1, Rabbit – (Cat# RA22119) http://bit.ly/1lEw98H Organization: Colorado State UniversityKetul Popat - Jul 25, 2014 - Rating: 5.0
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